منابع مشابه
Determination of receptor specificities for whole influenza viruses using multivalent glycan arrays.
Influenza viruses bind to mucosal glycans to gain entry into a host organism and initiate infection. The target glycans are often displayed in multivalent arrangements on proteins; however, how glycan presentation influences viral specificity is poorly understood. Here, we report a microarray platform approximating native glycan display to facilitate such studies.
متن کاملImproved spot morphology for printed glycan arrays.
Despite considerable success studying glycan-binding proteins using printed glycan arrays (PGAs), unambiguous quantitation of spot intensities by fluorescent readers remains a challenge. The main obstacles are the varying spot shape and size and in-spot fluorescence distribution caused by uneven drying of the printed drops. Two methods have been suggested for solving this problem: using polymer...
متن کاملA General Synthetic Route to Defined, Biologically Active Multivalent Arrays
A new, general strategy for the synthesis of biologically active multivalent arrays displaying diverse functionality has been developed. This method exploits the ability of ring-opening metathesis polymerizations to produce polymers of defined lengths. By incorporating N-hydroxysuccinimide esters into the polymers, recognition epitopes bearing nucleophilic functional groups, in this case an R-m...
متن کاملGlycan arrays for analysis of influenza virus specificity
Approaches that improve upon the pharmacokinetic and pharmacodynamic properties of singlestrand antisense oligonucleotides are an active research topic. Antisense oligonucleotide phosphorothioates (ASOs) are highly protein bound. Therefore, it is likely that they traffic into and through cells via a protein to protein shuttling mechanism driven by binding affinity gradients.1 The ASO binding ev...
متن کاملDissecting Multivalent Lectin–Carbohydrate Recognition Using Polyvalent Multifunctional Glycan-Quantum Dots
Multivalent protein-carbohydrate interactions initiate the first contacts between virus/bacteria and target cells, which ultimately lead to infection. Understanding the structures and binding modes involved is vital to the design of specific, potent multivalent inhibitors. However, the lack of structural information on such flexible, complex, and multimeric cell surface membrane proteins has of...
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ژورنال
عنوان ژورنال: Faraday Discussions
سال: 2019
ISSN: 1359-6640,1364-5498
DOI: 10.1039/c9fd00080a